Liudmila Samsonova is the defending champion from last year and will open up against Danielle Collins.
2023 Mubadala Citi DC Open ATP and WTA Prize Money and Points Breakdown with $2,013,940 on offer
Other top ties include Caroline Garcia who is the second seed facing the winner of Marta Kostyuk - Bianca Andreescu.
Jessica Pegula is the top seeed and could face Sofia Kenin. Elina Svitolina will again face Victoria Azarenka in a repeat of their Wimbledon clash.
Madison Keys also faces Qinwen Zheng, recent Palermo winner with Jennifer Brady and Anhelina Kalinina also in the same section.