The draw has been released with Tsitsipas is top seed and will face off against Grigor Dimitrov who recently returned to form at Indian Wells.
Gael Monfils will face Lorenzo Musetti in a tie to watch, while Casper Ruud will hope to continue his momentum towards Turin when he faces Lloyd Harris.
Jannik Sinner is seventh seed and will take on Reilly Opelka in another top tie.
Hubert Hurkacz is set to take on Andy Murray with Carlos Alcaraz, the winner of last night's Red Bull Bassline set to face Dan Evans in the same section.
Matteo Berrettini will face a qualifier, while recent Indian Wells winner, Cameron Norrie will return to action against Marton Fucsovics.
Alexander Zverev finally is set to take on Filip Krajinovic in his opener as second seed.
2021 Erste Bank Open Vienna Draw