The 2019 US Open champion, Bianca Andreescu, and women's world number 1, Ashleigh Barty won't take part in this year's US Open. On the other, the 23-time Grand Slam champion Serena Williams will have a chance to win her record-breaking 24th Grand Slam title.

World number 3, Karolina Pliskova will be the first-seeded in Flushing Meadows this year. She will play against Anhelina Kalinina in the first round. The second-seeded Sofia Kenin will meet Yanina Wickmayer. Serena Williams will be the third-seeded at the 2020 US Open despite being the ninth highest-ranked woman in the WTA Rankings. She will take on Kristie Ahn in the first round.